Viral im Yasni Exposé von Gunter Kraus

Guenter Kraus. SINT-KATELIJNE-WAVER, BE. 1. 20100035229 METHODS, PLASMID VECTORS AND PRIMERS FOR ASSESSING HIV VIRAL FITNESS 02-11-2010 ...

T-cell lines (Jurkat and Molt-4) were transduced with retroviral vectors containing a hairpin ribozyme that targets a conserved sequence in the 5' transcribed leader sequence of human immunodeficiency virus (HIV) type 1.

We have previously described a hairpin ribozyme that targets a highly conserved sequence in the U5 region of HIV-1. To determine if escape mutations would compromise virus replication, we introduced critical mutations into the ribozyme target site of an infectious molecular clone of HIV-1MN.

Infrequent parenteral dosing could facilitate maintenance therapy or prophylactic use of antiretrovirals (ARV). TMC278, a next-generation NNRTI, has demonstrated potent sustained activity against HIV-1 after once-daily oral doses as hydrochloride salt. We have investigated the pharmacokinetics and tolerability after intramuscular and subcutaneous (SC) injections of TMC278 long-acting formulations.

Advances in genetic analysis and a greater understanding of human immunodeficiency virus (HIV) molecular pathogenesis have identified critical viral targets for gene interference strategies.